![]() Southern blotting: It is the technique used for the identification of particular sequence of DNA. It is used to determine the expression of target protein.Western blotting is used for identification of different diseases like that of hepatitis B, BSE, HIV etc.Image Reference: Application of western blotting: It is again incubated in the room temperature for 2-3 hours and then TBST is added, and hence the resultant is visualized by different means either by the use of fluorescent process, radioactive detection etc.After the incubation process is over, membrane is washed for any unbound antibody and then secondary antibody is again added.It can be done from 30minutes to overnight. Primary antibody is then added and the mixture of antibody and membrane is incubated.Blocking of membrane is done using BSA and TBS for the non- specific binding of antibodies. ![]() Then the membrane is blocked so that antibodies bind only to the target protein.It can be either nitrocellulose paper or nylon sheath. Once the molecules are separated, then the transfer of molecule in a membrane is done.The complete process is carried under the influence of electric current. Smaller and lighter molecules travel faster and larger molecules remains behind. In the process of electrophoresis, molecules are separated by their size. This process is completed using electrophoresis. Firstly, separation of macromolecules is done.Western blotting procedure: Western blotting is completed using different steps. Submit all your requirements and you will soon get the quality work done by the professionals. All you need to do is, just relax and contact the professionals of this site. Experts in will complete your assignment quickly on the given topic. We are here to help you with your problem. But if you are finding it difficult to obtain all the information and frame a well- organized assignment on western blotting, dont get tensed. In order to make you perfect in this topic, teachers allot assignments and home works. This topic is important in any kind of research work. The key method used in the process of western blotting is the use of primary and secondary antibodies to find the target protein.Īssignments on western blotting are very common for science students. It takes place by the interaction of antibody with the antigen. Western blottingĭefinition of western blotting: It is the technique by which the identification of particular protein from the sample is done. Definition of blotting: Blotting means the transfer of sample that is obtained biologically from the gel to the membrane.
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